Anti-Neurofilament (NF-H) (Neuronal Marker) Recombinant Mouse Monoclonal Antibody (Clone:rNF421)

Catalogue Number: 12-1223-ABO

Manufacturer:Abeomics
Shelf Life:24 months
Type:Recombinant Monoclonal
Alias:NEFH; Neurofilament H; Neurofilament Heavy Polypeptide 200kDa; Neurofilament Triplet H Protein; NF-H; NF200
Shipping Condition:Blue Ice
Unit(s): 100 ug, 20 ug
Host name: Mouse
Clone: rNF421
Isotype: IgG1, kappa
Immunogen: Recombinant human neurofilament protein
Application: FACS, WB, IHC

Description

Description: This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Additional Text

Gene Name

NEFH

Gene ID

4744

Purification

Protein A/G Purified

Uniprot ID

P12036

Antibody Clonality

Monoclonal

Application Notes

Western Blot (1-2µg/ml); Flow Cytometry (1-2µg/million cells);Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Storage Note

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.