Anti-p53 Tumor Suppressor Protein Recombinant Mouse Monoclonal Antibody (Clone:rBP53-12)

Catalogue Number: 12-1231-ABO

Manufacturer:	Abeomics
Shelf Life:	24 months
Type:	Recombinant Monoclonal
Alias:	Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53
Shipping Condition:	Blue Ice
Unit(s):	100 ug, 20 ug
Host name:	Mouse
Clone:	rBP53-12
Isotype:	IgG1, kappa
Immunogen:	Recombinant full-length human wild-type TP53 protein
Application:	WB, IHC

Overview
Datasheets

Description

Description: This MAb reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.

Additional Text

Gene Name

TP53

Gene ID

7157

Uniprot ID

P04637

Purification

Protein A/G Purified

Antibody Clonality

Monoclonal

Application Notes

Western Blot (1-2µg/ml); Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Storage Note

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

12-1231 Datasheet

Price

£533.00

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