Anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Recombinant Mouse Monoclonal Antibody (Clone:rVWF/1465)

Catalogue Number: 12-1265-ABO

Manufacturer:Abeomics
Shelf Life:24 months
Type:Recombinant Monoclonal
Alias:Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)
Shipping Condition:Blue Ice
Unit(s): 100 ug, 20 ug
Host name: Mouse
Clone: rVWF/1465
Isotype: IgG1, kappa
Immunogen: Recombinant fragment of human vWF protein (aa 1815-1939) (exact sequence is proprietary)
Application: IP, WB, IHC

Description

Description: von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers althoµgh not all tumors of endothelial differentiation contain this antigen.

Additional Text

Gene Name

VWF

Purification

Protein A/G Purified

Gene ID

7450

Uniprot ID

P04275

Antibody Clonality

Monoclonal

Application Notes

Western Blot (1-2µg/ml); Immunoprecipitation (0.5-1ug/500ug protein lysate);Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Storage Note

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.