Phospho-Histone H2A.X (Ser139) (Clone: 1B3) rabbit mAb

Catalogue Number: 12-4094-ABO

Manufacturer:Abeomics
Type:Recombinant Monoclonal
Alias:H2AFX, H2AX, ?H2AX, gamma-H2AX
Shipping Condition:Blue Ice
Unit(s): 20 ul, 200 ul
Host name: Rabbit
Clone: HisH2AXS139-1B3
Isotype: IgG1, kappa
Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser139 of human phospho histone H2A.X.
Application: FACS, WB

Description

Description: Histone H2AX is a variant of the nucleosome core histone H2A and is phosphorylated at Ser139 in response to DNA damage. Histone H2AX phosphorylation is considered a specific reporter of double-strand DNA breaks. The protein is also referred to as gH2AX when phosphorylated at Ser139. H2AX phosphorylation is especially strong in response to double-strand breaks formed during apoptosis. However, physiological phosphorylation of Histone H2AX occurs when double-strand DNA breaks are formed during meiosis and V(D)J recombination. A549 and DU145 cell lines have been found to have higher expression levels of phosphorylated Histone H2AX compared to Jurkat, MCF-7, or HL-60 cell lines.

Additional Text

Antibody Clonality

Monoclonal

Application Notes

1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.(0.5mg/ml, more than 200 western blots)

Storage Note

Store at -20°C. Avoid repeated freeze and thaw cycles.