Cleaved PARP (Asp214) (Clone: H8) rabbit mAb

Catalogue Number: 12-4264-ABO

Manufacturer:Abeomics
Type:Recombinant Monoclonal
Alias:Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1
Shipping Condition:Blue Ice
Unit(s): 20 ul, 200 ul
Host name: Rabbit
Clone: PARP-H8
Isotype: IgG1, kappa
Immunogen: A synthetic peptide corresponding to residues surrounding Asp214 of human PARP
Application: FACS, WB

Description

Description: Poly-ADP-ribose polymerase 1 (PARP-1), is a substrate of caspase-3 and caspase-7, both of which play a dominant role in apoptosis. PARP is cleaved into 89 and 24 kDa fragments at Asp214. The detection of these fragments is used as an indicator of caspase activation and apoptosis induction for many cell lines. Under normal conditions, PARP aids in the detection and repair of DNA damage. With 1-2 million copies per nucleus, PARP is also involved in poly (ADP-ribosyl)ation, a post-translational protein modification mechanism used to modify chromatin structure and regulate transcription. Decreased PARP activity has been shown to lead to loss of memory and neuronal cell death.

Additional Text

Antibody Clonality

Monoclonal

Application Notes

1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.(0.5mg/ml, more than 200 western blots)

Storage Note

Store at -20°C. Avoid repeated freeze and thaw cycles.