Cleaved PARP (Asp214) (Clone: H8) rabbit mAb PE conjugate
Catalogue Number: 12-4265-ABO
| Manufacturer: | Abeomics |
| Type: | Recombinant Monoclonal |
| Alias: | Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 10 tests, 100 tests |
| Host name: | Rabbit |
| Clone: | PARP-H8 |
| Isotype: | IgG1, kappa |
| Immunogen: | A synthetic peptide corresponding to residues surrounding Asp214 of human PARP |
| Application: | FACS |
Description
Description: Poly-ADP-ribose polymerase 1 (PARP-1), is a substrate of caspase-3 and caspase-7, both of which play a dominant role in apoptosis. PARP is cleaved into 89 and 24 kDa fragments at Asp214. The detection of these fragments is used as an indicator of caspase activation and apoptosis induction for many cell lines. Under normal conditions, PARP aids in the detection and repair of DNA damage. With 1-2 million copies per nucleus, PARP is also involved in poly (ADP-ribosyl)ation, a post-translational protein modification mechanism used to modify chromatin structure and regulate transcription. Decreased PARP activity has been shown to lead to loss of memory and neuronal cell death.
Additional Text
Antibody Clonality
Monoclonal
Application Notes
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Note
Store at 2-8°C. Avoid repeated freeze and thaw cycles.
12-4265 Datasheet
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