Alexa Fluor® 488-conjugated AffiniPure™ Fab Fragment Goat Anti-Alpaca IgG, VHH domain ( min X Bov Sr Prot)

Catalogue Number: 128-547-232-JIR

Manufacturer:	Jackson ImmunoResearch
Shelf Life:	12 months
Preservative:	0.05% Sodium azide
Physical state:	Freeze-dried solid
Type:	Polyclonal Secondary Antibody - Conjugated
Shipping Condition:	RT
Unit(s):	0.25 mg
Host name:	Goat
Clone:
Isotype:
Immunogen:	IgG, VHH domain

Overview
Datasheets

Description

Description: Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa. Based on immunoelectrophoresis, Western blot and/or ELISA, the antibody reacts with the VHH domain of heavy chain (HC) alpaca IgG, subclasses 2 and 3; and with the VHH domain of llama IgG, subclasses 2 and 3. It also reacts with whole molecule alpaca and llama IgG1. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine serum proteins, but it may cross-react with immunoglobulins from other species.

Additional Text

Antibody Clonality

Polyclonal

Short Description

Goat Anti-Alpaca IgG, VHH domain secondary antibody, conjugated to Alexa Fluor® 488. This is an affinity purified Fab Fragment polyclonal antibody and has been tested and/or adsorbed to ensure minimal cross reactivity with Bovine serum proteins.

Application Notes

1:100 - 1:800 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.