Catalogue Number: 128-625-232-JIR
Manufacturer: | Jackson ImmunoResearch |
Preservative: | 0.05% Sodium azide |
Physical state: | Freeze-dried solid |
Type: | Polyclonal Secondary Antibody - Conjugated |
Shipping Condition: | RT |
Unit(s): | 0.25 mg |
Host name: | Goat |
Clone: | |
Isotype: | |
Immunogen: | IgG, VHH domain |
Description: Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis, Western blot and/or ELISA, the antibody reacts with the VHH domain of heavy chain (HC) alpaca IgG, subclasses 2 and 3; and with the VHH domain of llama IgG, subclasses 2 and 3. It also reacts with whole molecule alpaca and llama IgG1. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine serum proteins, but it may cross-react with immunoglobulins from other species.
Polyclonal
Western Blot:- 1:50,000-200,000 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.