Histone H3K9me1 Antibody, SNAP-Certified™ for CUT&RUN and ChIP
Catalogue Number: 13-0029-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 12 months |
| Physical state: | Aff. Pur. IgG |
| Type: | ChIP Validated Antibodies |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | A synthetic peptide corresponding to histone H3 monomethylated at lysine 9. |
| Application: | ICC, ChIP, WB, CUT&RUN |
Description
Description: This antibody meets EpiCypher's lot-specific SNAP-Certified™ criteria for specificity and efficient target enrichment in both CUT&RUN and ChIP applications. In CUT&RUN, this requires <20% cross-reactivity to related histone PTMs determined using the SNAP-CUTANA™ K-MetStat Panel of spike-in controls (EpiCypher 19-1002, Figure 1). High target efficiency is confirmed by consistent genomic enrichment at 500k and 50k starting cells (Figures 2-3). In ChIP, this requires <20% cross-reactivity to related histone PTMs and >5% of target input recovered as determined using the SNAP-ChIP® K-MetStat Panel of spike-in controls (EpiCypher 19-1001, Figure 6) [1].
Additional Text
Purification
Affinity Purified
Modification
Monomethylated, Lys9
Antibody Clonality
Monoclonal
Application Notes
CUT&RUN: 0.5 µg per reaction ChIP: 2 - 5 µg per 5 µg chromatin IF/ICC: 0.5 - 2 µg/mL WB: 0.2 - 1 µg/mL
Storage Note
Stable for 1 year at -20°C from date of receipt.
13-0029 Datasheet
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