H3K4me3 Antibody, SNAP-Certified™ for CUT&RUN
Catalogue Number: 13-0041-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 12 months |
| Physical state: | Aff. Pur. IgG |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Host Cell: | Rabbit|Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | A synthetic peptide corresponding to histone H3 trimethylated at lysine 4 |
| Application: | ICC, IF, WB, CUT&RUN |
Description
Description: This H3K4me3 (histone H3 lysine 4 trimethyl) antibody meets EpiCypher's lot-specific SNAP-Certified™ criteria for specificity and efficient target enrichment in CUT&RUN. This requires <20% cross-reactivity to related histone PTMs determined using the SNAP-CUTANA™ K-MetStat Panel of spike-in controls (EpiCypher 19-1002, Figure 1). High target efficiency is confirmed by consistent genomic enrichment at 500k and 50k starting cells (Figures 2-4). This antibody targets histone H3 trimethylated at lysine 4, which is enriched at active promoters near transcription start sites (TSS). *Mixed Monoclonal: a pool of multiple monoclonal antibodies.
Additional Text
Purification
Affinity Purified
Antibody Clonality
Monoclonal
Application Notes
CUT&RUN: 0.5 µg per reaction Western Blot: 1:250 Immunofluorescence: 1:100
Storage Note
Stable for 1 year at -20°C from date of receipt
13-0041 Datasheet
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