SNF2L/SMARCA1 CUTANA™ CUT&RUN Antibody

Catalogue Number: 13-2005-EPC

Manufacturer:EpiCypher
Shelf Life:12 months
Physical state:Aff. Pur. IgG
Type:Polyclonal Primary Antibody - Unconjugated
Host Cell:Rabbit|Antibody
Shipping Condition:Blue Ice
Storage Condition:2-8°C
Unit(s): 100 ul
Host name: Rabbit
Clone:
Isotype:
Immunogen: Between amino acids 1004 and 1054
Application: IP, IHC, CUT&RUN

Description

Description: This antibody meets EpiCypher's CUTANA Compatible criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols (epicypher.com/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. SNF2L/SMARCA1 antibody produces CUT&RUN peaks above background (Figure 1) that overlap with H3K4me3 (Figures 1-2), consistent with its known role as the ATP-dependent helicase subunit of the NURF ISWI chromatin remodeler complex [1].

Additional Text

Purification

Affinity Purified

Antibody Clonality

Polyclonal

Application Notes

CUT&RUN 0.5 µg per reaction Immunoprecipitation 2 - 10 µg/mg lysate Immunohistochemistry 1:1,000 - 1:5,000 Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections

Storage Note

Stable for 1 year at 4°C from date of receipt