Catalogue Number: 13-2016-EPC
Manufacturer: | EpiCypher |
Shelf Life: | 12 months |
Physical state: | Aff. Pur. IgG |
Type: | Polyclonal Primary Antibody - Unconjugated |
Host Cell: | Rabbit|Antibody |
Shipping Condition: | Blue Ice |
Storage Condition: | 2-8°C |
Unit(s): | 100 ul |
Host name: | Rabbit |
Clone: | |
Isotype: | |
Immunogen: | Between amino acids 25 and 75 |
Application: | IP, WB, CUT&RUN |
Description: This antibody meets EpiCypher's "CUTANA Compatible" criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols (epicypher.com/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. CHD4 is a member of the nucleosome remodeling and deacetylase (NuRD) complex. Within the complex, CHD4 remodels chromatin by deacetylating histones [1]. CHD4 antibody produces CUT&RUN peaks above background (Figure 1) within gene promoters, intergenic, and intronic regions (Figures 1-2).
Affinity Purified
Polyclonal
CUT&RUN 0.5 µg per reaction Western Blot 1:10,000 - 1:25,000 Immunoprecipitation 2-10 µg/mg lysate
Stable for 1 year at 4°C from date of receipt