EGFR CUTANA™ CUT&RUN Antibody

Catalogue Number: 13-2018-EPC

Manufacturer:	EpiCypher
Shelf Life:	12 months
Preservative:	0.09% sodium azide
Ph:	7-8
Physical state:	Aff. Pur. IgG
Type:	Polyclonal Primary Antibody - Unconjugated
Host Cell:	Rabbit\|Antibody
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	100 ul
Host name:	Rabbit
Clone:
Isotype:
Immunogen:	Between amino acids 1100 and 1150
Application:	WB, IHC, CUT&RUN

Overview
Datasheets

Description

Description: This antibody meets EpiCypher's "CUTANA Compatible" criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols (epicypher.com/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. EGFR is a tyrosine kinase receptor for epidermal growth factor (EGF) and other ligands of the EGF family. Activated EGFR can carry out its biological effects via the cytoplasmic pathway or nuclear pathway. The cytoplasmic pathway can cause tumorigenesis, metastasis, high proliferation, and resistance to chemotherapy and radiation.The nuclear pathway causes membrane EGFR to translocate into the nucleus and interact with other DNA-binding transcription factors, which can activate gene expression [1]. EGFR antibody produces CUT&RUN peaks above background (Figure 1) within gene promoters, intronic, and intergenic regions (Figures 1-2).

Additional Text

Purification

Affinity Purified

Antibody Clonality

Polyclonal

Application Notes

CUT&RUN 0.5 µg per reaction Western Blot 1:10,000 - 1:50,000 Immunohistochemistry 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections

Storage Note

Stable for 1 year at 4°C from date of receipt

13-2018 Datasheet

Price

£432.00

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