Menin CUTANA™ CUT&RUN Antibody

Catalogue Number: 13-2021-EPC

Manufacturer:	EpiCypher
Shelf Life:	12 months
Preservative:	0.09% sodium azide
Ph:	7-8
Physical state:	Aff. Pur. IgG
Type:	Polyclonal Primary Antibody - Unconjugated
Host Cell:	Rabbit\|Antibody
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	100 ul
Host name:	Rabbit
Clone:
Isotype:
Immunogen:	Between amino acids 575 and 615
Application:	IP, WB, IHC, CUT&RUN

Overview
Datasheets

Description

Description: This antibody meets EpiCypher's "CUTANA Compatible" criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols (epicypher.com/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. Menin antibody produces CUT&RUN peaks above background primarily in promoter and intronic regions (Figure 1) that overlap with H3K4me3 (Figure 2), consistent with its known role as a component of MLL/SET1 histone methyltransferase complex, where it specifically methylates H3K4. As a part of the MLL/SET1 complex, Menin mediates its tumor suppressor activity by regulating histone methylation of HOX and CDK inhibitor genes [1].

Additional Text

Purification

Affinity Purified

Antibody Clonality

Polyclonal

Application Notes

CUT&RUN 0.5 µg per reaction Immunoprecipitation 2 - 10 µg/mg lysate Immunohistochemistry 1:500 - 1:2,000 Western Blot 1:10,000 - 1:25,000 Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections

Storage Note

Stable for 1 year at 4°C from date of receipt

13-2021 Datasheet

Price

£432.00

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