Buccutite™ Rapid Protein Crosslinking Kit *Microscale Optimized for Crosslinking 100 ug Antibody Per Reaction*
Catalogue Number: 1315-AAT
| Manufacturer: | AAT Bioquest |
| Type: | Conjugation Kit |
| Shipping Condition: | Blue Ice |
| Unit(s): | 2 labelings |
Description
Description: Protein-protein conjugations are commonly performed with a bifunctional linker such as SMCC. One end of the SMCC reacts (via NHS ester) with amines (-NH2) found in the amino acid lysine and N-terminus, and the other end reacts (via maleimide) with the thiol groups (-SH) found in the amino acid cysteine. However, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause significant amount of homo-crosslinking. In addition it is quite difficult and tedious to quantify the number of maleimide groups on a protein. AAT Bioquest has developed a convenient and effective crosslinking method to link two biomolecules with a high conjugation yield. Our method uses one pair of crosslinkers: Buccutite™ MTA and Buccutite™ FOL. MTA is added to one protein, while FOL is added to another protein. Protein-protein cross-linking reaction is initiated by mixing Protein-1-Buccutite ™ MTA and Protein-2-Buccutite ™ FOL. This crosslinking reaction occurs under extremely mild neutral conditions without any catalyst required, and it is robust and efficient.
Additional Text
Storage Note
Multiple;|6 months
1315 MSDS
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations