Catalogue Number: 1321-AAT
Manufacturer: | AAT Bioquest |
Shelf Life: | 6 months |
Type: | Conjugation Kit |
Shipping Condition: | Blue Ice |
Unit(s): | 2 Labelings |
Description: APC-Cy7 is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at ~780 nm. AAT Bioquest offers this Buccutite™ rapid labeling kit to facilitate the APC-Cy7 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Buccutite™ APC-Cy7 Tandem Conjugation Kit provides a robust and convenient method to conjugate antibodies with APC. The kit includes a preactivated APC-Cy7 tandem and reaction buffer. The conjugated antibody can be used in WB, ELISA and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 100 ug of antibody. The best ratio for any new antibody reagent must be determined by experimentation. Our kit provides preactivated APC-Cy7 tandem to facilitate the APC-Cy7 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-Cy7 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-Cy7 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
Refrigerated (2-8 °C); Minimize light exposure;|6 months