Ac-LEHD-AMC *CAS 292633-16-0*

Catalogue Number: 13426-AAT

Manufacturer:AAT Bioquest
Shelf Life:12 months
Type:Enzyme Substrates
Shipping Condition:RT
Unit(s): 5 mg

Description

Description: Caspase 9 is a member of the CED-3 subfamily of the caspase family of cysteine proteases that play an essential role in the execution phase of apoptosis. These enzymes share a dominant primary specificity for cleaving bonds following aspartic acid residues. "Initiator" caspases (such as caspase 8) activate "effector" caspases, such as caspases 3 and 7. The effector caspases then cleave cellular substrates ultimately leading to the morphological changes of apoptosis. Ac-LHED-AMC is a selective fluorogenic substrate for caspase 9. The caspase 9-induced hydrolysis of Ac-LHED-AMC results in the release of AMC fluorophore that is detected using an excitation wavelength of ~365 nm and an emission wavelengh of ~450 nm. The assay can be run in the assay buffer consisting of 50 mM MES, pH 6.5, 10% PEG 8000, 0.1% CHAPS, 5 mM DTT, and 1 mM EDTA.

Additional Text

Storage Note

Freeze (< -15 °C); Minimize light exposure;|12 months

CAS Number

292633-16-0