Casein, TAMRA-conjugated
Catalogue Number: 13441-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Protein - Conjugated |
| Shipping Condition: | RT |
| Unit(s): | 5 mg |
Description
Description: Casein is considered to be a generic substrate for a broad spectrum of proteases. As native casein this fluoresceinated casein is hydrolyzed by many proteases, and widely used for fluorometric measurement of protease activity. In the intact substrate, casein is heavily labeled with TAMRA, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly green fluorescent dye-labeled short peptides. The increase in fluorescence intensity is directly proportional to protease activity. Compared to FITC-labeled casein, this casein substrate has pH-independent fluorescence. This feature is more convenient for the assays that require low pH. We do not recommend that this conjugate be used for fluorescence polarization assay. For fluorescence polarization we can custom-make the lightly labeled fluorescein casein conjugate.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
13441 MSDS
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