mFluor™ Violet 450-VAD-FMK
Catalogue Number: 13475-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Fluorescent Reagents |
| Shipping Condition: | RT |
| Unit(s): | 25 Tests |
Description
Description: FAM-VAD is a blue fluorescent cell-permeable polycaspase inhibitor to target caspases 1, 2, 3, 6, 8, 9, or 10. Once inside the cell, the inhibitor binds covalently to the active caspase, which produces a green fluorescence. When added to a population of cells, the FAM-VAD-FMK probe enters each cell and covalently binds to a reactive cysteine residue that resides on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. The bound labeled reagent is retained within the cell, while any unbound reagent will diffuse out of the cell and is washed away. The green fluorescent signal is a direct measure of the amount of active caspase present in the cell at the time the reagent was added. Cells that contain the bound labeled reagent can be analyzed by 96-well plate-based fluorometry, fluorescence microscopy, or flow cytometry. The probe has the spectral properties similar to Pacific Blue® and BD Horizon™ V450, thus the filter set of Pacific Blue® and BD Horizon™ V450 can be conveniently used with a flow cytometer or microscope. This probe is particularly designed for the multicolor detection of caspases with a violet laser-equipped flow cytomer.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
13475 Protocol
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