Anti-Nestin Antibody
Catalogue Number: 1435-NES-PPS
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Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (cat. 1435-NES, red, 1:500) and anti-MAP2 antibody (cat. 1100-MAP2, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
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Immunostaining of cultured neonatal rat neurons and glia stained with anti-nestin antibody (cat. 1435-NES, red, 1:500) and anti-vimentin antibody (cat. 2105-VIM, green, 1:500). The blue is Hoechst staining for nuclear DNA. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
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Immunolabeling of the coronal section of an embryonic day 15.5 mouse cortex showing specific labeling of Nestin (cat. 1435-NES, 1:500; green). Nuclei stained with DAPI (blue). Image kindly provided by Alyssa Treptow, University of California, Riverside.
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Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 kDa nestin doublet.
1435-NES Image
Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (cat. 1435-NES, red, 1:500) and anti-MAP2 antibody (cat. 1100-MAP2, green,1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
1435-NES Image
Immunostaining of cultured neonatal rat neurons and glia stained with anti-nestin antibody (cat. 1435-NES, red, 1:500) and anti-vimentin antibody (cat. 2105-VIM, green,1:500). The blue is Hoechst staining for nuclear DNA. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
1435-NES Image
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 kDa nestin doublet.
| Manufacturer: | Phosphosolutions |
| Shelf Life: | 12 months |
| Preservative: | 5 mM Sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | ESTM 46 antibody, FLJ 21841 antibody, FLJ21841 antibody, Intermediate filament protein antibody, Nbla00170 antibody, nes antibody, NEST_HUMAN antibody, Nestin antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | 4D11 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant human Nestin expressed in and purifeid from E. coli. |
| Application: | ICC, WB, IHC |
Additional Text
Gene Name
NES
Gene ID
10763
Purification
Protein G purified
Uniprot ID
P48681
Antibody Clonality
Monoclonal
Molecular Weight
220-240
Storage Note
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Short Description
Our Anti-Nestin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Nestin and is Protein G purified. It is great for use in WB, IHC, ICC.
1435-NES Datasheet
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we offer free delivery to UK universities and non profit organisations