Anti-Neurofilament NF-H Antibody
Catalogue Number: 1451-NFH-PPS
1451-NFH Image
1451-NFH Image
1451-NFH Image
1451-NFH Image
1451-NFH Image
1451-NFH Image
1451-NFH Image
1451-NFH Image
1451-NFH Image
1451-NFH Image
1451-NFH Image
Immunostaining of mixed cultured rat neurons and glia stained with anti-NFH antibody (cat. 1451-NFH, red, 1:25,000) and anti-NFL antibody (cat. 1452-NFL, green, 1:100).
1451-NFH Image
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament H (cat. 1451-NFH, 1:25,000, red) in nuclei of neurons and specific labeling of GFAP (cat. 620-GFAP, 1:5000, green) in astrocytes and other glial cells, and DAPI staining of nuclear DNA.
1451-NFH Image
Immunofluorescence of a section of rat cerebellum showing specific labeling of the axons with anti-neurofilament H antibody (cat. 1451-NFH, green, 1:25,000). The section is colabeled with anti-calbindin (cat. 302-CALB , red, 1:1000) labeling the dendrites of Purkinje cells. The blue is DAPI staining nuclear DNA.
1451-NFH Image
Immunofluorescence of a section of adult rat frontal cortex labeled with anti-Neurofilament-M (cat. 1454-NFM, 1:5000, red) and anti-Neurofilament-H (cat. 1451-NFH, 1:5000, green). The anti-NFM specifically labels neuron cell bodies and dendrites of pyramidal neurons, while the anti-NFH labels the network of neuronal axons only. The blue is Hoechst staining of nuclear DNA.
1451-NFH Image
Immunofluorescence of a section of rat spinal cord labeled with anti-UCHL1 (cat. 2060-UCHL1, 1:500, red) and anti-Neurofilament H (cat. 1451-NFH, 1:25,000, green). The large cells are alpha-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites.
1451-NFH Image
Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.
1451-NFH Image
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament H (cat. 1451-NFH, 1:25,000, red) in nuclei of neurons and specific labeling of GFAP( cat. 620-GFAP , 1:5000, green) in astrocytes and other glial cells, and DAPI staining of nuclear DNA.
1451-NFH Image
Immunostaining of mixed cultured rat neurons and glia stained with anti-NFH antibody (cat. 1451-NFH, red, 1:25,000) and anti-NFL antibody (cat. 1452-NFL, green, 1:100).
1451-NFH Image
Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.
1451-NFH Image
Immunofluorescence of a section of rat cerebellum showing specific labeling of the axons with anti-neurofilament H antibody (cat. 1451-NFH, green, 1:25,000). The section is colabeled with anti-calbindin ( cat. 302-CALB , red, 1:1000) labeling the dendrites of Purkinje cells. The blue is DAPI staining nuclear DNA.
| Manufacturer: | Phosphosolutions |
| Preservative: | 10 mM Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | Purified bovine NF-H |
| Application: | ICC, WB, IHC |
Additional Text
Gene Name
NEFH
Gene ID
4744
Uniprot ID
P12036
Molecular Weight
200
Antibody Clonality
Polyclonal
Short Description
Our Anti-Neurofilament NF-H primary antibody from PhosphoSolutions is chicken polyclonal. It detects all known mammal and chicken Neurofilament NF-H and is total IgY fraction. It is great for use in WB, IHC, ICC.
Specificity
200 kDa neurofilament protein antibody, CMT2CC antibody, Nefh antibody, Neurofilament heavy polypeptide 200kDa antibody, Neurofilament heavy polypeptide antibody, Neurofilament triplet H protein antibody, NF H antibody, NF-H antibody, NFH antibody, NFH_HUMAN antibody
Storage Note
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
1451-NFH Datasheet
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