ACF Chromatin Remodeling Enzyme Complex
Catalogue Number: 15-1013-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Other Enzymes |
| Host Cell: | Recombinant baculovirus in Sf9 cells/Recombinant insulin, Yes (S. cerevisiae) |
| Shipping Condition: | Dry Ice |
| Unit(s): | 100 reactions |
Description
Description: Recombinant Drosophila ACF Chromatin Remodeling Enzyme Complex, assembled from Acf1-FLAG and ISWI produced in SF9 cells. ACF is an ATP-dependent chromatin remodeling complex that regulates nucleosome spacing.
Additional Text
Protein Tag
6xHIS-FLAG™ Tag
Application Notes
This product is sufficient to perform 100 remodeling reactions using EpiDyne®-FRET substrate. A single reaction is defined as 10 µL containing [5 nM ACF Complex, 20 nM EpiDyne®-FRET (EpiCypher 16-4201), 50 µM rATP] and remodels to completion in <20 minutes. 5x ACF Remodeling Assay Buffer is included (Catalog No. 21-0013; 100 mM Tris HCl pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20). For more information, see the EpiDyne®-FRET Technote (https://www.epicypher.com/resources/technical-notes/) or contact techsupport@epicypher.com
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
15-1013 Datasheet
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