SMARCA4 Chromatin Remodeling Enzyme
Catalogue Number: 15-1014-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Other Enzymes |
| Host Cell: | SF9 cells|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 100 reactions |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Full length recombinant human SMARCA4 Chromatin Remodeling Enzyme. SMARCA4 is a core ATPase subunit of the ATP-dependent chromatin remodeling complex SWI/SNF that mobilizes nucleosomes and plays key roles in gene transcription regulation, development, genome replication and repair [1,2]. Aberrant activity of SWI/SNF subunits is linked with more than 20% of human cancers and multiple developmental disorders, making SMARCA4 an emerging candidate therapeutic target [3,4]. SMARCA4 can be used in conjunction with EpiDyne®-FRET Remodeling Assay Substrate (EpiCypher 16-4201) in HTS-compatible drug discovery studies.
Additional Text
Protein Tag
6xHIS Tag
Application Notes
This product is sufficient to perform 100 remodeling reactions using EpiDyne®-FRET substrate (EpiCypher 16-4201). A single reaction is defined as 10 µL containing 5 nM SMARCA4 (concentration is lot-specific), 20 nM EpiDyne®-FRET, & 1 mM rATP with remodeling to completion in <30 minutes. Remodeling readout can be observed in the change of Cy3/Cy5 ratio over time in a black 384-well assay plate on a compatible fluorescent plate reader. 5x SMARCA Remodeling Assay Buffer is included (EpiCypher 21-0014; 100 mM Tris HCl pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20). See the EpiDyne®-FRET Technical Note (epicypher.com/resources/technical-notes) or contact techsupport@epicypher.com for more information.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
15-1014 Datasheet
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