CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows
Catalogue Number: 15-1016-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 12 months |
| Molecular Weight: | 44kDa |
| Ph: | 7.5 |
| Type: | Nucleases |
| Host Cell: | E. coli|S. aureus |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 reactions |
Description
Description: CUTANA™ pAG-MNase is the key reagent for performing Chromatin Immunocleavage (ChIC) [1] and Cleavage Under Targets and Release Using Nuclease (CUT&RUN) [2,3]. As a fusion of Proteins A and G to Micrococcal Nuclease, CUTANA pAG-MNase is compatible with target antibodies from various host species and is highly purified to remove contaminating E. coli DNA, which can complicate analysis at low cell numbers. This enzyme enables efficient mapping of chromatin features in ChIC/CUT&RUN, allowing for significant improvements in signal to noise and sequencing depth compared to ChIP-seq.
Additional Text
Protein Tag
6xHIS Tag
Application Notes
Add 2.5 µL of the supplied enzyme to a 50 µL CUT&RUN reaction (20X dilution). For more, see epicypher.com/protocols.
Storage Note
Stable for one year at -20°C from date of receipt
15-1016 Datasheet
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