CUTANA™ Non-Hot Start 2X PCR Master Mix for CUT&Tag
Catalogue Number: 15-1018-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | PCR Mixes |
| Host Cell: | E. coli|Polymerase |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 reactions |
Description
Description: CUTANA Non-Hot Start 2X PCR Master Mix for CUT&Tag is specifically formulated to amplify next generation sequencing (NGS) libraries from CUTANA™ pAG-Tn5 tagmented DNA generated during CUT&Tag (EpiCypher 15-1017) [1]. This ready-to-use master mix only requires the addition of primers and the tagmented chromatin to achieve high fidelity amplification of NGS libraries.
Additional Text
Protein Tag
Untagged
Application Notes
Recommended use: For detailed instructions regarding non-hot start PCR cycling conditions in CUT&Tag, see CUTANA™ Direct-to-PCR CUT&Tag protocol: epicypher.com/protocols NOTE: Not recommended for use with primers or templates containing uracil.
Storage Note
Stable for 6 months at -20°C from date of receipt. The master mix should be completely thawed on ice and resuspended prior to use.
15-1018 Datasheet
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