CpG ODN (C792), TLR9 ligand (Class C)
Catalogue Number: 15-1023-ABO
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Fig-1: Induction of human TLR9 activity by CpG ODN C792.
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Fig-2: Induction of mouse TLR9 activity by CpG ODN C792.
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Fig-3: Abeomics' CpG ODNs did not show any TLR2 agonist activity. TLR2/NF-kB Leeporter™ HEK293 cells (14-127ACL) were treated with various CpG ODNs at 100 ug/ml as well as a positive TLR2 agonist, Pam3CSK4, at 10 ng/ml for 16 h, and luciferase activity was then analyzed.
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Fig-4: Abeomics' CpG ODNs did not show any TLR4 agonist activity. TLR4/IL-8 Leeporter™ HeLa cells (14-124ACL) were treated with various CpG ODNs at 100 ug/ml as well as a positive TLR4 agonist, LPS, at 10 ng/ml for 16 h, and luciferase activity was then analyzed.
| Manufacturer: | Abeomics |
| Shelf Life: | 6 months |
| Type: | Ligand |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg, 1 mg |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Application: | FA |
Description
Description: Sequence: 5'-tCGaaCGttCGaaCGttCGaaCGttCGaat-3' (Class C ODN C792: Bases are phosphorothioate.) Synthetic oligodeoxynucleotides (ODN) containing unmethylated deoxycytosine-deoxyguanosine (CpG) motif are equivalent to bacterial DNA in the immunostimulatory activity, which can induce innate immunity via Toll-like receptor 9 (TLR9) in mammals. There are three major classes of CpG ODNs, and each ODN class exhibits different stimulatory effects on immune cell activation. Class A ODNs are potent inducers of IFN-alpha that leads to the plasmacytoid dendritc cell (pDC) maturation. Class B ODNs are relatively weak inducers of type I IFNs but strong stimulators of human B cells and monocyte maturation. Class C ODNs combine elements of both Classes A and B ODNs, which can induce IFN-alpha in pDC and activation of B cells.
Additional Text
Application Notes
A. CpG ODN C792-mediated human TLR9 activation in TLR9/NF-kB Leeporter™ - HEK293 cells (Figure 2). 1. Harvest TLR9/NF-kB Leeporter™ - HEK293 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 5 x 104 cells/well. 2. Incubate cells at 37°C in a CO2 incubator for overnight. 3. The next day, stimulate cells with various amounts of CpG ODN C792. 4. Incubate at 37°C in a CO2 incubator for 6-16 hours. 5. Add 30-50 ul of luciferase assay reagent per well. 6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer. B. CpG ODN C792-mediated mouse TLR9 activation in NF-kB Leeporter™ - RAW 264.7 cells (Figure 3). 1. Harvest NF-kB Leeporter™ - RAW 264.7 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 5 x 104 cells/well. 2. Incubate cells at 37°C in a CO2 incubator for overnight. 3. The next day, stimulate cells with various amounts of CpG ODN C792. 4. Incubate at 37°C in a CO2 incubator for 6-16 hours. 5. Add 30-50 ul of luciferase assay reagent per well.
Storage Note
Upon receipt, store at -20°C (Stable for at least 6 months). Avoid frequent freeze/thaw cycles.
15-1023 Datasheet
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