CUTANA™ Uncharged pAG-Tn5 for CUT&Tag
Catalogue Number: 15-1025-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 12 months |
| Ph: | 7.2 |
| Type: | Other Enzymes |
| Host Cell: | E. coli|E. coli |
| Shipping Condition: | Blue Ice |
| Unit(s): | 20 ul |
Description
Description: Products in EpiCypher's IDEA Toolbox (Innovation and Discovery of Epigenetic Applications) offer access to reagents without known or fully defined uses, enabling researchers to explore cutting-edge applications. Due to their novelty and unexplored potential, EpiCypher will engage in limited technical support. CUTANA Uncharged pAG-Tn5 is a fusion of Proteins A and G to E. coli transposase (Tn5) and is the key enzyme for CUT&Tag [1]. This product is highly purified to remove contaminating E. coli DNA. For superior normalization, as well as antibody validation and reaction monitoring, SNAP-CUTANA™ nucleosome spike-ins (e.g. EpiCypher 19-1002) are recommended. This Tn5 is uncharged and must be loaded with user-designed mosaic adapter DNA prior to use in CUT&Tag. The uncharged protein exists as a monomer but will dimerize when charged with DNA. For a charged enzyme that is ready to use, see EpiCypher 15-1017.
Additional Text
Protein Tag
Untagged
Application Notes
pAG-Tn5 transposomes can be assembled as previously described [1]. In brief, 3.2 µL of an equimolar mixture of pre-annealed user-defined Adapter-A and user-defined Adapter-B oligonucleotides (50 µM each, 100 µM total adapter DNA) should be mixed with 20 µL of 5.5 µM pAG-Tn5 fusion protein dimer (a 3:1 molar ratio of adapter DNA to pAG-Tn5 dimer). The mixture is then incubated on a rotating platform for 1 hour at room temperature and stored at -20°C. Specific activity definition of the charged pAG-Tn5 is highly recommended before use in CUT&Tag. Due to the confounding variable of user-supplied mosaic adapters, EpiCypher will not engage in protocol troubleshooting for this reagent. For a pre-charged enzyme that is eligible for technical support, see EpiCypher 15-1017.
Storage Note
Stable for one year at -20°C from date of receipt. The protein is not subject to freeze/thaw under these conditions.
15-1025 datasheet
SUPPORT
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