CUTANA™ Fluorescent pAG-Tn5 for CUT&Tag
Catalogue Number: 15-1026-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 12 months |
| Ph: | 7.2 |
| Type: | Other Proteins |
| Host Cell: | E. coli|E. coli |
| Shipping Condition: | Blue Ice |
| Unit(s): | 125 ul |
Description
Description: Products in EpiCypher's IDEA Toolbox (Innovation and Discovery of Epigenetic Applications) offer access to reagents without known or fully defined uses, enabling researchers to explore cutting-edge applications. Due to their novelty and unexplored potential, EpiCypher will engage in limited technical support. CUTANA Fluorescent pAG-Tn5 is a fusion of Proteins A and G to E. coli transposase (Tn5), the key enzyme for CUT&Tag [1]. The Mosaic A and Mosaic B adapters loaded in this enzyme bear a 5' Cy5 fluorescent dye to enable multiomic visualization and mapping of chromatin features. This product is highly purified to remove contaminating E. coli DNA. For an enzyme charged with non-fluorescent adapters that can be used in standard CUT&Tag, see EpiCypher 15-1017.
Additional Text
Protein Tag
Untagged
Application Notes
A 20X dilution of this reagent is sufficient for a standard CUT&Tag reaction following the EpiCypher CUTANA™ Direct-to-PCR CUT&Tag protocol (epicypher.com/protocols), indicating that addition of the fluorescent dye does not prevent Tn5-mediated tagmentation of chromatin (see Figures 2 & 3). However, application-specific optimization will be necessary for use in microscopy. Due to the novelty of this reagent, EpiCypher will not engage in application tech support.
Storage Note
Stable for one year at -20°C from date of receipt. The protein is not subject to freeze/thaw under these conditions.
15-1026 Datasheet
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