CUTANA™ pAG-Tn5 for CUT&Tag
Catalogue Number: 15-1117-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 12 months |
| Type: | Other Enzymes |
| Host Cell: | E. coli|E. coli |
| Shipping Condition: | Blue Ice |
| Unit(s): | 250 Reactions |
Description
Description: CUTANA pAG-Tn5 is the key reagent for performing efficient mapping of chromatin features in Cleavage Under Targets and Tagmentation (CUT&Tag) [1]. CUT&Tag offers significant improvements in signal to noise at reduced cell inputs and sequencing depth compared to ChIP-seq. As a fusion of Proteins A and G to a highly active E. coli transposase mutant (Tn5), CUTANA pAG-Tn5 is compatible with target antibodies from a broad spectrum of host species. It also comes without an epitope tag, making it suitable for tag-mediated CUT&Tag (e.g. FLAG, HA, TY1, V5, etc.). This product is highly purified to remove contaminating E. coli DNA, which can be tagmented during storage and complicate analysis at low cell numbers. For superior normalization as well as antibody validation and reaction monitoring, SNAP-CUTANA™ nucleosome spike-ins (e.g. EpiCypher 19-1002) are recommended. The Tn5 comes charged with mosaic adapters and is ready to be used in CUT&Tag.
Additional Text
Protein Tag
Untagged
Application Notes
Add 2.5 µL of the supplied enzyme to a 50 µL CUT&Tag reaction (20X dilution). For detailed applications and uses of this product, please see epicypher.com/protocols.
Storage Note
Stable for one year at -20°C from date of receipt. The protein is not subject to freeze/thaw under these conditions.
15-1117 Datasheet
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