Histone Octamer, Recombinant Human
Catalogue Number: 16-0001-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | DNA Binding Proteins |
| Host Cell: | E.coli |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Human histone octamers (two each of histones H2A, H2B, H3 and H4) are made from recombinant histones expressed in E. coli. Octamers are the protein component of nucleosomes, the basic subunit of chromatin. Assembling this product with DNA bearing a 601 Widom sequence (e.g. EpiCypher 18-0005, 18-0006) produces a nucleosome. The 601 Widom sequence, originally discovered by Lowary and Widom [1], has high affinity for octamers.
Additional Text
Protein Tag
Untagged
Application Notes
Human recombinant histone octamers can be used for chromatin reconstitution experiments or as substrates for histone modifying enzymes. See Luger et al. [2] for recommended nucleosome reconstitution protocol. Histone octamers should not be stored under conditions where the salt concentration is lower than 800 mM. Please see lot-specific Technical Datasheets for product concentration.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
16-0001 Datasheet
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