HeLa Mononucleosomes, Purified
Catalogue Number: 16-0002-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | HeLa cells |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Human mononucleosomes purified from HeLa cells. The nucleosome is the basic repeating unit of chromatin, which consists of 150 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3, and H4).
Additional Text
Protein Tag
Untagged
Application Notes
HeLa Mononucleosomes are suitable for use in enzyme assays such as acetylation or methylation, chromatin binding, or as a positive control in western blotting. Use 1-2 µg per reaction. Molarity and molecular weight are estimated based on DNA size and account for the endogenous post-translational histone modifications. Note: despite the high purity of the preparation, a small amount of arginine methyltransferase (RMTase) activity may co-purify with the nucleosomes. If you are studying weak or low turnover HMTases, you can use AMI-1 to inhibit the endogenous RMTase activity.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws
16-0002 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations