HeLa Polynucleosomes, Purified
Catalogue Number: 16-0003-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | HeLa cells |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Human polynucleosomes purified from HeLa cells. The nucleosome is the basic subunit of chromatin, which consists of 150 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3 and H4). HeLa Polynucleosomes are predominantly trimers, with some dimers and tetramers (Figure 1).
Additional Text
Application Notes
HeLa Polynucleosomes are suitable for use in enzyme assays such as acetylation or methylation, chromatin binding, or as a positive control in western blotting. Use 1-2 µg per reaction. Molarity and molecular weight are estimated based on DNA size and account for the endogenous post-translational histone modifications. Note: despite the high purity of the preparation, a small amount of arginine methyltransferase (RMTase) activity may co-purify with the nucleosomes. If you are studying weak or low turnover HMTases, you can use AMI-1 to inhibit the endogenous RMTase activity.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws
16-0003 Datasheet
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