Mononucleosomes (H3.3), Human Recombinant
Catalogue Number: 16-0012-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Shipping Condition: | Dry Ice |
| Unit(s): | 100 ug |
Description
Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.3-P84243; H4-P62805) wrapped by 147 base pairs of 601 sequence DNA. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly.
Additional Text
Application Notes
Recombinant mononucleosomes are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. EpiCypher Mononucleosomes (H3.3), Human Recombinant do not contain free DNA which could alter assayed activities.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
16-0012 Datasheet
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