Mononucleosomes (H3.3), Human Recombinant

Catalogue Number: 16-0012-EPC

Manufacturer:EpiCypher
Shelf Life:6 months
Type:Nucleosome
Shipping Condition:Dry Ice
Unit(s): 100 ug

Description

Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.3-P84243; H4-P62805) wrapped by 147 base pairs of 601 sequence DNA. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly.

Additional Text

Application Notes

Recombinant mononucleosomes are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. EpiCypher Mononucleosomes (H3.3), Human Recombinant do not contain free DNA which could alter assayed activities.

Storage Note

Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.