Mononucleosomes, Recombinant Human, Desthiobiotinylated
Catalogue Number: 16-0024-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.1, and H4 Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom [1], is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' desthiobiotin-TEG group. Desthiobiotin has a weaker affinity (Kd = 10-11) for avidin-family proteins than does biotin (Kd = 10-15).
Additional Text
Application Notes
Mononucleosomes, Human Recombinant Desthiobiotinylated are highly purified and suitable for use as substrates in enzyme screening assays, structural studies, or effector protein binding experiments. The weaker affinity of desthiobiotin for avidin-family proteins allows nucleosomes to be eluted via addition of free biotin. The ability for these nucleosomes to be recovered intact is useful in many aspects of chromatin biology.
Storage Note
Stable for six (6) months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
16-0024 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations