Nucleosome, Recombinant Human, H3K4me3 dNuc, Biotinylated

Catalogue Number: 16-0316-EPC

Manufacturer:EpiCypher
Shelf Life:6 months
Type:Nucleosome
Host Cell:E. coli & synthetic DNA|Human
Shipping Condition:Dry Ice
Unit(s): 50 ug, 20 ug
Host name: Human
Clone:
Isotype:
Immunogen:

Description

Description: Recombinant mononucleosomes (H3K4me3) consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.2, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. H3K4me3 dNuc contains trimethyl-lysine at position 4 on histone H3.2. H3K4me3 has a Cys to Ala substitution at position 110. The DNA contains a 5' biotin-TEG group.

Additional Text

Modification

Trimethylated, Lys4

Application Notes

H3K4me3 mononucleosome is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments.

Storage Note

Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws