Mononucleosomes (H3.3G34V), Recombinant Human Biotinylated
Catalogue Number: 16-0347-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3, accession P84243) containing a valine at position 34, expressed in E. coli. Histone H3 is one of the four proteins that are present in the nucleosome, the basic repeating unit subunit of chromatin, consisting of 147 base pairs of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). H3.3 is a histone variant, a non-allelelic replacement histone found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). The substitution of V for G at position 34 is associated with pediatric diffuse intrinsic pontine gliomas (DIPGs) with and diminished levels of H3K36 methylation. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5' biotin-TEG group.
Additional Text
Uniprot ID
P84243
Gene ID
3020; 3021
Application Notes
Mononucleosomes (H3.3G34V), Recombinant Human are highly purified and are suitable for use as substrates in enzyme screening assays, structural studies or for effector protein binding experiments. Mononucleosomes (H3.3G34V), Recombinant Human from EpiCypher do not contain free DNA which could alter assayed activities.
Gene Name
H3-3A, H3-3B
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
16-0347 Datasheet
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