Mononucleosomes (H3.3G34W), Recombinant Human Biotinylated
Catalogue Number: 16-0348-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Oncogenic mononucleosomes (oncoNucs) consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.3, H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. H3.3 is a histone variant, a non-allelelic replacement histone found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). The substitution of W for G at position 34 is associated with pediatric diffuse intrinsic pontine gliomas (DIPGs) and diminished levels of H3K36 methylation. The DNA in this nucleosome contains a 5' biotin-TEG group.
Additional Text
Application Notes
H3.3G34W oncoNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
16-0348 Datasheet
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