Mononucleosomes (H3.3K4M), Recombinant Human Biotinylated
Catalogue Number: 16-0349-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.3 and H4; accession numbers: H2A-P04908, H2B-O60814, H3.3-P84243, H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA, identified by Lowary and Widom [1]. H3.3 is a histone variant, found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3) contains a lysine-to-methionine (K-to-M) substitution at position four, a known cancer-associated oncohistone mutation that affects chromatin regulatory mechanisms (Nacev et al., 2018). The 601 sequence DNA contains a 5' biotin-TEG group, enabling affinity binding applications.
Additional Text
Application Notes
H3.3K4M mononucleosomes are highly purified and are suitable for a variety of applications, including use as a substrate in enzymatic assays or for effector protein binding experiments.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
16-0349 Datasheet
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