Mononucleosomes (H3.3K4M), Recombinant Human Biotinylated

Catalogue Number: 16-0349-EPC

Manufacturer:EpiCypher
Shelf Life:6 months
Type:Nucleosome
Shipping Condition:Dry Ice
Unit(s): 50 ug

Description

Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.3 and H4; accession numbers: H2A-P04908, H2B-O60814, H3.3-P84243, H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA, identified by Lowary and Widom [1]. H3.3 is a histone variant, found in regions of high chromatin turnover outside of S-phase (e.g. at actively transcribed genes). Recombinant human histone H3.3 (H3F3A, H3.3A, H3F3) contains a lysine-to-methionine (K-to-M) substitution at position four, a known cancer-associated oncohistone mutation that affects chromatin regulatory mechanisms (Nacev et al., 2018). The 601 sequence DNA contains a 5' biotin-TEG group, enabling affinity binding applications.

Additional Text

Application Notes

H3.3K4M mononucleosomes are highly purified and are suitable for a variety of applications, including use as a substrate in enzymatic assays or for effector protein binding experiments.

Storage Note

Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.