Nucleosome, Recombinant Human, H3K27ac dNuc Biotinylated
Catalogue Number: 16-0365-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug, 25 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Recombinant mononucleosomes (H3K27ac) consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of histones H2A, H2B, H3.2 and H4) to form a nucleosome, the basic repeating unit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. H3K27ac dNuc contains acetyl-lysine at position 27 on histone H3.2. H3K27ac has a Cys to Ala substitution at position 110. The DNA contains a 5' biotin-TEG group.
Additional Text
Modification
Acetylated, Lys27
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
Application Notes
H3K27ac dNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzymatic assays or for effector protein binding experiments.
16-0365 Datasheet
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