H3K18ac Recombinant Nucleosome, Biotinylated
Catalogue Number: 16-0372-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Histone acetylation is a post-translational modification (PTM) wherein an acetyl group is added to a lysine residue of a histone protein. In combination with other PTMs, histone acetylation constitutes the "histone code," acting as a language read by proteins to regulate chromatin structure and gene expression. Histone acetylation is associated with gene activation and binds reader proteins such as SWI/SNF (BAF) chromatin remodeling complexes, BRD4, and TAF1 [1]. Recombinant nucleosomes containing acetylated histones are useful to study the biological functions of histone acetylation. H3K18ac (histone H3 lysine 18 acetylation) Recombinant Nucleosome, Biotinylated consists of 147 base pairs of 601 sequence DNA [2] wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3.2, and H4) to form a nucleosome, the basic repeating unit of chromatin. The DNA contains a 5' biotin-TEG group. H3K18ac nucleosome contains H3.2 with acetylated lysine at position 18. Histone H3.2 also contains a Cys to Ala substitution at position 110. H3K18 is acetylated by lysine acetyltransferases (KATs) such as KAT3A (CBP) and KAT3B (p300), and H3K18ac is deacetylated by lysine deacetylases (KDACs) such as SIRT7 and HDAC1 [1].
Additional Text
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
Application Notes
H3K18ac nucleosome is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments. For a corresponding unmodified control, we recommend EpiCypher 16-0006.
16-0372 Datasheet
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