Nucleosome, Recombinant Human, H3K4,9,14,18ac/H4K5,8,12,16ac dNuc, Biotinylated
Catalogue Number: 16-0374-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histones (created by proprietary semi-synthetic methods) contain acetyl-lysine at H3 positions 4, 9, 14, and 18 and H4 positions 5, 8, 12, and 16. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5' biotin-TEG group. *H3K4,9,14,18ac has a Cys to Ala substitution at position 110.
Additional Text
Modification
Acetylated, Lys4, H3
Application Notes
H3K4,9,14,18ac/H4K5,8,12,16ac dNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzymatic assays or for effector protein binding experiments.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
16-0374 Datasheet
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