Nucleosome, Recombinant Human, H4K20ac dNuc, Biotinylated
Catalogue Number: 16-0377-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: EpiCypher dNucs™ are semi-synthetic recombinant nucleosomes, containing one or more histone post translational modifications (PTMs). Nucleosomes, the basic repeating unit of chromatin, are the natural targets of chromatin readers and modifying enzymes, providing a physiologically relevant substrate for characterization of epigenetic proteins. dNucs consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4). The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. H4K20ac dNuc is acetylated at position 20 on histone H4. The DNA in this nucleosome contains a 5'biotin-TEG group.
Additional Text
Modification
Acetylated, Lys20
Application Notes
H4K20ac dNuc is highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
16-0377 Datasheet
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