Mononucleosomes (H2AX), Human Recombinant
Catalogue Number: 16-1013-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2AX, H2B, H3 and H4. Accession numbers: H2AX-P16104; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. The 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. H2AX is a variant of histone H2A that is phosphorylated on serine 139 by the ATM and ATR kinases in response to DNA double strand breakage [2]. Phosphorylation of H2AX serves to recruit a number of DNA damage repair proteins, such as BRCA1 and NBS1 [2].
Additional Text
Application Notes
H2AX Mononucleosomes are highly purified and suitable for a variety of applications, including use as a substrate in enzymatic assays or for effector protein binding experiments.
Storage Note
Stable for six (6) months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
16-1013 Datasheet
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