Nucleosome, Recombinant Human, Acidic Patch Mutant H2AE92K
Catalogue Number: 16-1030-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Ph: | 7.5 |
| Type: | Nucleosome |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
Description
Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A*, H2B, H3 and H4; accession numbers: H2A-P04908*; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA [1]. *Histone H2A contains a glutamate-to-lysine (E-to-K) substitution at position 92 (H2AE92K). H2AE92 is among key residues forming a negatively charged region on the nucleosome surface named the "acidic patch". The acidic patch is a conserved interaction hub for neighboring nucleosomes and nucleosome binding proteins, often via salt bridges with arginine anchors, and is functionally critical in chromatin condensation and chromatin remodeling [2-4]. H2AE92 resides in the H2A C-terminal extension and is associated with nucleosome binding factors such as histone H4 N-terminal tail, LANA, RCC1, IL-33, SIR3, HMGN2, and remodeling ATPases [2-4]. H2AE92K disrupts binding with SMARCB1 [5].
Additional Text
Application Notes
H2AE92K mononucleosomes are highly purified and suitable for a variety of applications to test the effect of acidic patch mutation on enzymatic activity or chromatin binding. See EpiCypher 16-0030 for a biotinylated version of this mutant.
Storage Note
Stable for six (6) months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
16-1030 Datasheet
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