Mononucleosomes (H3.3K27M), Recombinant Human, Biotinylated
Catalogue Number: 16-1323-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E. coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: Oncogenic Nucleosomes (oncoNucs™) are recombinant nucleosomes assembled using histones with cancer-associated mutations, providing physiologically relevant substrates for cancer research. OncoNucs consist of 147 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.3 and H4). The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. H3.3K27M mononucleosome contains a methionine at position 27 on histone H3.3, which is a dominant negative inhibitor of the EZH2 methyltransferase of the PRC2 complex and associated with pediatric brain cancer (DIPG). The DNA in this nucleosome contains a 5' biotin-TEG group.
Additional Text
Application Notes
Mononucleosomes (H3.3K27M) are highly purified and suitable for a variety of applications, including use as a substrate in enzyme assays, high-throughput screening and inhibitor testing, chromatin binding studies, protein-protein interaction assays, structural studies, and in effector protein binding experiments.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
16-1323 Datasheet
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