EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, Biotinylated
Catalogue Number: 16-4111-EPC
| Manufacturer: | EpiCypher |
| Shelf Life: | 6 months |
| Type: | Nucleosome |
| Host Cell: | E.coli & synthetic DNA|Human |
| Shipping Condition: | Dry Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | |
| Isotype: | |
| Immunogen: |
Description
Description: EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1 consists of 217 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 217 base pair DNA sequence includes an upstream 6 bp linker, the 145 bp ST601 nucleosome positioning sequence, identified by Lowary and Widom [1], and a downstream 66 bp linker (hereby named 6-N-66 nucleosome). One copy of the 'GATC' DNA motif is embedded within the ST601 sequence and there is a 5' biotin-TEG group. The terminally positioned nucleosome is a suitable target for recognition and substrate for remodeling reactions by various chromatin interacting proteins.
Additional Text
Application Notes
This product is a substrate for nucleosome remodeling assays that can monitor the accessibility of the GATC site (in bold, below). The GATC motif is masked by the nucleosome's octamer core in its native configuration and exposed to enable access by enzymes such as DpnII or dam methyltransferase after remodeling.
Storage Note
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.
16-4111 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations