iFluor® 546-streptavidin conjugate
Catalogue Number: 16958-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Avidin/Streptavidin Biotin Products |
| Shipping Condition: | RT |
| Unit(s): | 200 ug |
Description
Description: Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This iFluor™ 546-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with iFluor™ 546 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. iFluor™ 546-streptavidin conjugates has fluorescence excitation and emission maxima essentially identical to those of Alexa Fluor® 546 conjugates. These spectral characteristics make it an excellent alternative to Alexa Fluor® 546-streptavidin conjugate (Alexa Fluor® is the trademark of Invitrogen). It is a very valuable tool for biotin-streptavidin-based biological assays and tests. Under the same conditions we tested, iFluor™ 546-streptavidin conjugate is brighter and more photostable than the corresponding Alexa Fluo® 546 conjugate. These spectral and labeling characteristics make the iFluor™ 546 dye family a superior alternative to Alexa Fluor® 546.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
16958 Protocol
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