Histamine Research EIA (96 wells), "RUO"
Catalogue Number: 17-HISRT-E01-RES-ALP
| Manufacturer: | Alpco Diagnostics |
| Type: | Other ELISA Kits |
| Alias: | Histamine |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 96 Wells |
| Host name: | |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Range: | 0.3 - 30 ng/mL |
| Sensitivity: | 0.2 ng/mL |
| Sample type: | Plasma |
| Sample size: | 10 µL |
| Application: | IA, EIA |
Description
Description: During the sample preparation, Histamine is quantitatively acylated. The subsequent competitiveResearch Histamine ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls, and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.
Additional Text
Regulatory Statuses
Research Use Only. Not for Use in Diagnostic Procedures.
Short Description
Research Histamine ELISA
Storage Note
store at 2-8C
17-HISRT-E01-RES Protocol
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations