Biotin-20-dUTP *1 mM in TE Buffer (pH 7.5)*
Catalogue Number: 17018-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 6 months |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Dry Ice |
| Unit(s): | 25 nmoles |
Description
Description: The biotin-modified deoxyuridine 5'-triphosphates are widely used for a variety of non-radioactive DNA labeling reactions including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. The biotinylated probes have been shown to hybridize to homologous nucleic acid at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probes can be detected by avidin and streptavidin. Biotin-20-dUTP can be enzymatically incorporated into DNA via nick-translation, random priming, 3'-end terminal labeling or in the process of PCR. Biotin-20-dUTP is used to produce biotinylated DNA probes in a variety of hybridization applications including Southern blots, Northern blots, dot blots, fixed cells and tissues.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|6 months
17018 MSDS
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