Propidium iodide *10 mM aqueous solution*
Catalogue Number: 17517-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 6 months |
| Type: | Fluorescent Reagents |
| Shipping Condition: | RT |
| Unit(s): | 1 mL |
Description
Description: Propidium iodide (PI) belongs to the same chemical class of ethidium bromide. As in the case of ethidium bromide its fluorescence is enhanced for 20-30-fold upon binding to nucleic acids. The fluorescence excitation maximum is red-shifted for 30–40 nm and the fluorescence emission maximum blue-shifted for 15 nm or so. PI also binds to RNA as DAPI and acridine orange. PI is commonly used for identifying dead cells in a population of cells and as a counterstain in multicolor fluorescent techniques. It can also be used to differentiate necrotic, apoptotic and normal cells. It is suitable for fluorescence microscopy, flow cytometry and fluorometry.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|6 months
17517 Protocol
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